EuroFlow BCP-ALL MRD 2025
Resources
Detailed information
EuroFlow SOPs and relevant documents
EuroFlow SOP for bulk lysis in MRD panels. Version 1.3 - June 2018
EuroFlow SOP for instrument set-up and compensation for DxFlex. Version 1.2 - July 2023
EuroFlow SOP for instrument set-up and compensation for BD LSR II and BD FACS Canto. Version 1.2.1 - October 2019
EuroFlow SOP for instrument set-up and compensation for BD Lyric for 8-color panels, Version 1.8 – 1 July 2019
EuroFlow SOP for instrument set-up and compensation for BD LSRFortessa X-20. Version 2.2 - September 2024
EuroFlow SOP for instrument set-up and compensation for Navios. Version 1.5.1 - October 2019019
Composition and technical info of EuroFlow panels for hematological malignancies. Version 1.11 - November 2023
List of reference and alternative antibodies for hematological malignancies panels. Version 1.13 - November 2023
Uniform coding of EuroFlow panels. Version 1.1 - March 2019
Downloads for BD Lyric (if applicable):
Reagent files (zip) (download and extract)
Assay files (zip) (download and extract)
Label file (xml)
The above documents can be accessed after creating a EuroFlow account and upon login at app.euroflow.org/login
Videos
Educational video: EuroFlow SOP for instrument setup and compensation (for BD LSR II and BD FACS Canto)
euroflow.org
Publications
Theunissen et al., Standardized flow cytometry for highly sensitive MRD measurements in B-cell acute lymphoblastic leukemia. Blood. 2017 Jan 19;129(3):347-357.
pubmed.ncbi.nlm.nih.gov
Kalina et al., Quality assessment program for EuroFlow protocols: summary results of four-year (2010-2013) quality assurance rounds. Cytometry A. 2015 Feb;87(2):145-56.
pubmed.ncbi.nlm.nih.gov
Kalina et al., Frequent issues and lessons learned from EuroFlow QA. J Immunol Methods. 2019 Dec;475:112520.
pubmed.ncbi.nlm.nih.gov
Nováková et al., How to make usage of the standardized EuroFlow 8-color protocols possible for instruments of different manufacturers. J Immunol Methods. 2019 Dec;475:112388.
pubmed.ncbi.nlm.nih.gov
Glier et al., Comments on EuroFlow standard operating procedures for instrument setup and compensation for BD FACS Canto II, Navios and BD FACS Lyric instruments. J Immunol Methods. 2019 Dec;475:112680.
pubmed.ncbi.nlm.nih.gov